|Enrichment and Mass Spectrometry|
A phosphopeptide enrichment kit aids in processing protein digests and strong cation exchange fractions to selectively recover phosphopeptides for mass spectrometric analysis in less than 30 minutes. The Pierce Fe-NTA Phosphopeptide Enrichment Kit supplies high-capacity Fe-NTA spin columns and optimized buffer for analyzing 30 protein digests. The phosphopeptide-specific resin in each spin column will sufficiently enrich up to 150 micrograms of phosphopeptides. The spin columns complement other Thermo Scientific lysis, reduction, alkylation, and digestion reagents and graphite spin columns to provide a complete workflow for phosphopeptide enrichment.