When DNA targets high in GC content are amplified, PCR product formation can be compromised by inadequate strand separation and the propensity for complex secondary structure formation. The use of standard 7-deaza-dGTP is a method for overcoming this problem. CleanAmp 7-deaza-dGTP is a fusion of the secondary structure reducing nucleotide analog 7-deaza-dGTP and the manufacturer’s CleanAmp dNTP Hot Start technology. The product is available individually for amplification of routine GC-rich targets or as the CleanAmp 7-deaza-dGTP mix, which is recommended for more challenging targets with higher GC content.
TriLink BioTechnologies Inc.